More footage has emerged allegedly showing Australian livestock being abused overseas.
This time, welfare group Animals Australia says it has proof that Australian sheep are being inhumanely handled and killed in the notorious Al Rai meat market in Kuwait.
The Al Rai market is not approved to receive Australian sheep under the Federal Government's new ESCAS welfare system for live exports.
The Department of Agriculture has launched an investigation, which is expected to take some weeks. DAFF says it's the responsibility of the exporter to ensure its animals do not end up outside approved supply chains.
In the footage, one sheep appears to have its throat cut with a knife 24 times.
ESCAS, or the Exporter Supply Chain Assurance System, was introduced after Animals Australia released footage of Australian cattle being abused in Indonesian abattoirs.
That led to a month-long suspension of the live cattle trade in June last year.
Ninety-nine per cent of all the foreign markets for Australian livestock must now have ESCAS-approved abattoirs and supply chains in place. It is illegal for an exporter to send livestock into an unapproved supply chain such as the Al Rai market.
Despite repeated requests for an interview, the Minister for Agriculture, Senator Joe Ludwig, has been unavailable to speak with the ABC.
Government backbencher Kelvin Thompson says he thinks responsibilty for the oversight of welfare in the live export trade should be taken away from DAFF and given to the Department of Health instead.
"It is a matter of concern for me that it's taken the efforts of Animals Australia to bring these breaches to the attention of the government."
Animals Australia campaign director Lyn White says the system has failed.
"This is the scene of some of the worst cruelty that I've documented to Australian sheep over the past nine years," she said.
"We were appalled to discover up to 200 sheep were being openly sold at this market, in breach of the new Australian regulations.
"The exporter should be investigated and, if found guilty of these breaches, the strongest possible penalty should be imposed."
Meanwhile, a newspaper in Pakistan is reporting its federal ports minister wants an investigation into how Australian sheep, some infected with scabby mouth, were allowed to be unloaded in Karachi.
An Australian boat carrying 22,000 head of sheep rejected in Bahrain unloaded in the city on Thursday, Australian time.
The Pakistan Observer reports the ship wasn't scheduled to travel to Pakistan and an inquiry will be held by the department of ports and shipping
An Australian Government-accredited veterinarian says conditions on live export boats are not of a sound standard.
Lloyd Reeve-Johnson worked as a vet for the Australian Quarantine and Inspection Service on live exports boats between 2006 and 2009.
He says vets are paid by the exporting company, which can sacrifice independence, and animals are often treated inhumanely by inexperienced crews.
"Multiply to that, you're in conditions which are often sub-optimal temperatures which might reach the forties, humidities which may be up in the ninetieth percentile, rough and rolling seas, poor ventilation," he said.
"All of these things make a ship journey extremely stressful.
"It's not just the 1 per cent or 2 per cent who die, it's the 99 per cent who are just having a terrible time."
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